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polyclonal serum against inca (anti-rabbit)  (Gramsch Inc)

 
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    Gramsch Inc polyclonal serum against inca (anti-rabbit)
    (A) HeLa cells were infected with Ctr- EB (MOI-1) for 14, 20 and 26 h and cells were analyzed via FACS as . (B) HeLa cells were infected with Ctr- EB for 14, 24 and 36 h. Input (5%) was taken after homogenization step (before plasma membrane protein isolation) and subjected to WB analysis to determine pEphA2, Hsp60 and Actin. The blot was stripped and reprobed for total EphA2. Numbers under the blot represents fold activation for pEphA2 with respect to total EphA2. (C) Plasma membrane protein was isolated from UN or Ctr -infected cells at different time points and subjected to WB analysis. Pan Cadherin was used as a control for plasma membrane. <t>IncA</t> was used as a marker to test the quality of the isolated plasma membrane. * Unspecific bands detected by IncA <t>polyclonal</t> serum. (D) Culture medium of the UN as well as time course Ctr- infected cells (as indicated) was collected and TCA precipitated. The precipitated lysates were subjected to WB analysis against pEphA2, total EphA2 and GP96. (E, F) HeLa cells were transfected with EphA2-pcDNA3 at 37°C and the cells were infected with Ctr -pIncA-flag for 24 h at 35°C. (E) Cells were fixed and stained against Flag (red), EphA2 (green) and DNA (blue). (F) Cells were fixed and stained against Flag (red), pEphA2 (green) and DNA (blue). Magnification is indicated in size bar.
    Polyclonal Serum Against Inca (Anti Rabbit), supplied by Gramsch Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+serum+against+inca+%28anti-rabbit%29/pmc04408118-59-0-8?v=Gramsch+Inc
    Average 90 stars, based on 1 article reviews
    polyclonal serum against inca (anti-rabbit) - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "EphrinA2 Receptor (EphA2) Is an Invasion and Intracellular Signaling Receptor for Chlamydia trachomatis"

    Article Title: EphrinA2 Receptor (EphA2) Is an Invasion and Intracellular Signaling Receptor for Chlamydia trachomatis

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1004846

    (A) HeLa cells were infected with Ctr- EB (MOI-1) for 14, 20 and 26 h and cells were analyzed via FACS as . (B) HeLa cells were infected with Ctr- EB for 14, 24 and 36 h. Input (5%) was taken after homogenization step (before plasma membrane protein isolation) and subjected to WB analysis to determine pEphA2, Hsp60 and Actin. The blot was stripped and reprobed for total EphA2. Numbers under the blot represents fold activation for pEphA2 with respect to total EphA2. (C) Plasma membrane protein was isolated from UN or Ctr -infected cells at different time points and subjected to WB analysis. Pan Cadherin was used as a control for plasma membrane. IncA was used as a marker to test the quality of the isolated plasma membrane. * Unspecific bands detected by IncA polyclonal serum. (D) Culture medium of the UN as well as time course Ctr- infected cells (as indicated) was collected and TCA precipitated. The precipitated lysates were subjected to WB analysis against pEphA2, total EphA2 and GP96. (E, F) HeLa cells were transfected with EphA2-pcDNA3 at 37°C and the cells were infected with Ctr -pIncA-flag for 24 h at 35°C. (E) Cells were fixed and stained against Flag (red), EphA2 (green) and DNA (blue). (F) Cells were fixed and stained against Flag (red), pEphA2 (green) and DNA (blue). Magnification is indicated in size bar.
    Figure Legend Snippet: (A) HeLa cells were infected with Ctr- EB (MOI-1) for 14, 20 and 26 h and cells were analyzed via FACS as . (B) HeLa cells were infected with Ctr- EB for 14, 24 and 36 h. Input (5%) was taken after homogenization step (before plasma membrane protein isolation) and subjected to WB analysis to determine pEphA2, Hsp60 and Actin. The blot was stripped and reprobed for total EphA2. Numbers under the blot represents fold activation for pEphA2 with respect to total EphA2. (C) Plasma membrane protein was isolated from UN or Ctr -infected cells at different time points and subjected to WB analysis. Pan Cadherin was used as a control for plasma membrane. IncA was used as a marker to test the quality of the isolated plasma membrane. * Unspecific bands detected by IncA polyclonal serum. (D) Culture medium of the UN as well as time course Ctr- infected cells (as indicated) was collected and TCA precipitated. The precipitated lysates were subjected to WB analysis against pEphA2, total EphA2 and GP96. (E, F) HeLa cells were transfected with EphA2-pcDNA3 at 37°C and the cells were infected with Ctr -pIncA-flag for 24 h at 35°C. (E) Cells were fixed and stained against Flag (red), EphA2 (green) and DNA (blue). (F) Cells were fixed and stained against Flag (red), pEphA2 (green) and DNA (blue). Magnification is indicated in size bar.

    Techniques Used: Infection, Homogenization, Clinical Proteomics, Membrane, Isolation, Activation Assay, Control, Marker, Transfection, Staining



    Similar Products

    polyclonal serum against inca (anti-rabbit)  (Gramsch Inc)
    90
    Gramsch Inc polyclonal serum against inca (anti-rabbit)
    (A) HeLa cells were infected with Ctr- EB (MOI-1) for 14, 20 and 26 h and cells were analyzed via FACS as . (B) HeLa cells were infected with Ctr- EB for 14, 24 and 36 h. Input (5%) was taken after homogenization step (before plasma membrane protein isolation) and subjected to WB analysis to determine pEphA2, Hsp60 and Actin. The blot was stripped and reprobed for total EphA2. Numbers under the blot represents fold activation for pEphA2 with respect to total EphA2. (C) Plasma membrane protein was isolated from UN or Ctr -infected cells at different time points and subjected to WB analysis. Pan Cadherin was used as a control for plasma membrane. <t>IncA</t> was used as a marker to test the quality of the isolated plasma membrane. * Unspecific bands detected by IncA <t>polyclonal</t> serum. (D) Culture medium of the UN as well as time course Ctr- infected cells (as indicated) was collected and TCA precipitated. The precipitated lysates were subjected to WB analysis against pEphA2, total EphA2 and GP96. (E, F) HeLa cells were transfected with EphA2-pcDNA3 at 37°C and the cells were infected with Ctr -pIncA-flag for 24 h at 35°C. (E) Cells were fixed and stained against Flag (red), EphA2 (green) and DNA (blue). (F) Cells were fixed and stained against Flag (red), pEphA2 (green) and DNA (blue). Magnification is indicated in size bar.
    Polyclonal Serum Against Inca (Anti Rabbit), supplied by Gramsch Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+serum+against+inca+%28anti-rabbit%29/pmc04408118-59-0-8?v=Gramsch+Inc
    Average 90 stars, based on 1 article reviews
    polyclonal serum against inca (anti-rabbit) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) HeLa cells were infected with Ctr- EB (MOI-1) for 14, 20 and 26 h and cells were analyzed via FACS as . (B) HeLa cells were infected with Ctr- EB for 14, 24 and 36 h. Input (5%) was taken after homogenization step (before plasma membrane protein isolation) and subjected to WB analysis to determine pEphA2, Hsp60 and Actin. The blot was stripped and reprobed for total EphA2. Numbers under the blot represents fold activation for pEphA2 with respect to total EphA2. (C) Plasma membrane protein was isolated from UN or Ctr -infected cells at different time points and subjected to WB analysis. Pan Cadherin was used as a control for plasma membrane. IncA was used as a marker to test the quality of the isolated plasma membrane. * Unspecific bands detected by IncA polyclonal serum. (D) Culture medium of the UN as well as time course Ctr- infected cells (as indicated) was collected and TCA precipitated. The precipitated lysates were subjected to WB analysis against pEphA2, total EphA2 and GP96. (E, F) HeLa cells were transfected with EphA2-pcDNA3 at 37°C and the cells were infected with Ctr -pIncA-flag for 24 h at 35°C. (E) Cells were fixed and stained against Flag (red), EphA2 (green) and DNA (blue). (F) Cells were fixed and stained against Flag (red), pEphA2 (green) and DNA (blue). Magnification is indicated in size bar.

    Journal: PLoS Pathogens

    Article Title: EphrinA2 Receptor (EphA2) Is an Invasion and Intracellular Signaling Receptor for Chlamydia trachomatis

    doi: 10.1371/journal.ppat.1004846

    Figure Lengend Snippet: (A) HeLa cells were infected with Ctr- EB (MOI-1) for 14, 20 and 26 h and cells were analyzed via FACS as . (B) HeLa cells were infected with Ctr- EB for 14, 24 and 36 h. Input (5%) was taken after homogenization step (before plasma membrane protein isolation) and subjected to WB analysis to determine pEphA2, Hsp60 and Actin. The blot was stripped and reprobed for total EphA2. Numbers under the blot represents fold activation for pEphA2 with respect to total EphA2. (C) Plasma membrane protein was isolated from UN or Ctr -infected cells at different time points and subjected to WB analysis. Pan Cadherin was used as a control for plasma membrane. IncA was used as a marker to test the quality of the isolated plasma membrane. * Unspecific bands detected by IncA polyclonal serum. (D) Culture medium of the UN as well as time course Ctr- infected cells (as indicated) was collected and TCA precipitated. The precipitated lysates were subjected to WB analysis against pEphA2, total EphA2 and GP96. (E, F) HeLa cells were transfected with EphA2-pcDNA3 at 37°C and the cells were infected with Ctr -pIncA-flag for 24 h at 35°C. (E) Cells were fixed and stained against Flag (red), EphA2 (green) and DNA (blue). (F) Cells were fixed and stained against Flag (red), pEphA2 (green) and DNA (blue). Magnification is indicated in size bar.

    Article Snippet: Polyclonal serum against IncA (anti-rabbit) was obtained through Gramsch laboratories.

    Techniques: Infection, Homogenization, Clinical Proteomics, Membrane, Isolation, Activation Assay, Control, Marker, Transfection, Staining